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This updated and expanded coloured manual is written in simple language and lucid style. Most of the exercises have been revised and updated. Each exercise provides updated background information, principle and procedural techniques used in the experiments to further facilitate student understanding and performance of microbiology procedures. Two new exercises dealing with medical science have been created. This manual consists of 146 multidisciplinary natured exercises arranged in 18 units in addition to the chapter on World of microorganisms: An introduction. Students, faculty members and professionals working in diverse areas of life sciences and medical sciences will certainly find this manual useful and a valuable tool. Unique Features • Each exercise has been written to be reasonably self-contained. • Background information discussing the theory and experimental design underlying the procedures used in the exercise. • In depth discussion of the principle involved. • Materials needed for performing the practical. • Detailed procedure outlining the specific instructions. • Expected results with interpretation profusely illustrated with coloured photographs/diagrams to help students to perform practicals with their utmost ease in quest of achieving hundred percent success. • Precautions needed for safety and successful completion of an exercise. • The often asked questions at the time of viva-voce examination.