Biotechnology, Immunology, Biotechniques & Biostatistics (PB)

The present book entitled “Biotechnology, Immunology,  Biotechniques and Biostatistics” is designed to meet the demand for a text book for paper II prescribed for B.Sc. Zoology Third Year in the new Unified Syllabus for a number of State Universities in India.  The book is divided in four Units provided in the syllabus for this paper.  Unit I on Biotechnology consists of Chapters 1-9, Unit II on Immunology consists of Chapter 10-18, Unit III on Biotechniques consists of Chapters 19-28 and the last Unit IV on Biostatistics consists of Chapters 29-36. Efforts have been made to faithfully cover the entire contents for this paper.

CONTENTS
Preface v
UNIT-I
1. Biotechnology : History, Definition and Scope 3
2. Recombinant DNA and Genetic Engineering 1. Cloning and Expression Vectors 15
3. Recombinant DNA and Genetic Engineering 2. Chimeric DNA, Probes and Genomic
/cDNA Libraries 33
4. Crop Biotechnology 1. Gene Transfer Methods in Plants 57
5. Crop Biotechnology 2. Transgenic Plants (Including Cisgenic Plants 73
6. Biotechnology in Medicine: (Including Pharmaceuticals; Insulin Production) 91
7. Biotechnology for Bioenergy and Biofuels 105
8. Microbial Biotechnology: 1. Fermentation Technology and Food Processing 117

10. Immunology: An Overview 145
11. Lymphatic System and Lymphoid Organs 157
12. B-Cells and Antibodies (Immunoglobulins) 171
13. T Cells and T-Cell Receptors 183
14. MHC/HLA Restriction 197
15. Tolerance of Autoimmunity, Autoimmune Diseases and Immunodeficiency Diseases
Including AIDS 209
16. Interferons, Vaccines and Prevention of HIV Infection 219
17. Hybridoma Technology and Monoclonal Antibodies (mAbs) 231
18. Monoclonals without Hybridoma and Antibody Engineering using Genetic
Manipulations 241
viii Biotechnology and Immunology
UNIT-III
19. Biological Techniques and Tools: An Overview 253
20. Microtomy for Histological Studies 259
21. Microscopy: Study of Cells and Subcellular Structures 267
22. Cell Fractionation and Centrifugation 281
23. Chromatography for Separation of Polar Compounds from a Mixture 287
24. Electrophoresis for Separation of Charged Molecules in an Electric Field 297
25. Spectrophotometry for Quantitative Estimations 309
26. pH Values and pH Meter 317
27. Recombinant DNA Technology and PCR 321
28. Some Other Techniques for Cell Study 327
UNIT-IV
29. Sampling and Presentation of Data 335
30. Measures of Central Tendency 349
31. Measures of Dispersion/Spread 355
32. Probability (Addition and Multiplication Laws) 361
33. Normal, Binomial and Poisson Distributions 365
34. Tests of Significance (Z Test, t-Test, c2 Test, F Test) 373
35. Bivariate Analysis 1. Correlation Analysis 383
36. Bivariate Analysis 2. Regression Analysis 389
viii Biotechnology, Immunology, Biotechnique

The author of this book, Professor PK Gupta has 60 years of experience in teaching and conducting research in the fields of Cytogenetics, Genetics and Crop Biotechnology. He has already published >500 papers, most of these in International Journals of  repute and successfully supervised ~80 PhD students, some them occupying important positions in India and abroad. Therefore, he is also known for generating meritoreous human resource. He is also known for writing at least two dozen text books in the subjects of Genetics, Biotechnology and related areas, published by Rastogi Publications, MEERUT and edited several volumes of reference books in the field of Plant Cytogenetics, publsihed by reputed publishers including Macmillan India, Elsevier (The Netherland) and Springer (Germany). His books are widely read not only in India, but also in may foreign countries. He is a fellow of all the four national academies in the field of Science and Agriculture (FNA, FASc, FNASI, FNAAS) in India and has been the recipient of many awards including the prestigious Birbal Sahni Gold Medal of the Indian Botaniocal Society.