Laboratory Manual of Microbiology and Biotechnology (Paperback)

ISBN: 9789387025493

Edition: 2nd Edtion

Author : K. R. Aneja

Year: 2018

Pages: 475

Size: 8.5 X 11

Publisher Name: MEDTECH

Rs. 0.00
Rs. 750.00

This updated and expanded coloured manual is written in simple language and lucid style. Most of the exercises have been revised and updated. Each exercise provides updated background information, principle and procedural techniques used in the experiments to further facilitate student understanding and performance of microbiology procedures. Two new exercises dealing with medical science have been created.

This manual consists of 146 multidisciplinary natured exercises arranged in 18 units in addition to the chapter on World of microorganisms: An introduction.

Students, faculty members and professionals working in diverse areas of life sciences and medical sciences will certainly find this manual useful and a valuable tool.

Unique Features

  • Each exercise has been written to be reasonably self-contained.
  • Background information discussing the theory and experimental design underlying the procedures used in the exercise.
  • In depth discussion of the principle involved.
  • Materials needed for performing the practical.
  • Detailed procedure outlining the specific instructions.
  • Expected results with interpretation profusely illustrated with coloured photographs/diagrams to help students to perform practicals with their utmost ease in quest of achieving hundred percent success.
  •  Precautions needed for safety and successful completion of an exercise.
  • The often asked questions at the time of viva-voce examination.


Dr. (Prof.) K. R. Aneja is the past President of the Mycological Society of India (2013-14), Recorder of the Plant Sciences Section of ISCA (2008 & 2009), Chancellor’s Nominee for teacher’s selection at Punjabi University, Patiala (Punjab), and is a well known Academician, Researcher and Author known globally. He served as Professor and Chairman, Department of Microbiology, Kurukshetra University, Kurukshetra for 11 years.

He taught in the Departments of Botany and Microbiology for 34 years at Kurukeshetra University, Kurukshetra. He has completed six major research projects sponsored by the UGC and Ministry of Environment and Forests, New Delhi and DFID, UK (on socio-economic impact and management of parthenium weed in India using indigenous fungal pathogens). Dr. Aneja and his research students worked on fungal taxonomy, enzymology, microbial decomposition, biopesticides, and antimicrobial activity of fungi, plants and organic compounds against human and plant pathogens.

He has attended many conferences and presented invited papers, and chaired technical sessions, and organised an International conference. He is a recipient of INSA-Royal Society Academic Exchange Fellowship and worked on protein profiling and PCR of thermophilic fungi at the University of Abertay, Dundee, Scotland. He is an active member of over 10 National and International societies and is a Fellow of 5 societies: Indian Botanical Society, Indian Phytopathological Society, Indian Mycological Society, Society for Biocontrol Advancement and Mycological Society of India. He is an awardee of Rashtriya Gaurav Award and Best Citizens of India Award.

Dr. Aneja has published over 170 research papers and reviews and authored/edited nine books and two manuals: “From Ethnomycology to Fungal Biotechnology” Kluwer Academic/Plenum Publishers, USA; “Glimpses in Plant Sciences” Pragati Prakashan, Meerut; “An Introduction to Mycology”, “A Textbook of Basic and Applied Microbiology”, “Fungal Diversity and Biotechnology”, “Experiments in Microbiology, Plant Pathology and Biotechnology”, “Fundamentals of Agricultural Microbiology”, and “Medical Microbiology”, New Age International, Publishers, New Delhi; “Modern Food Microbiology” and Crueger’s Biotechnology: A Textbook of Industrial Microbiology”, Scientific International (Pvt.) Ltd., New Delhi and “Frontiers in Food Microbiology” Nova Science Publishers, USA. 

His research interests include: Fungal Diversity, Environmental Microbiology, Agricultural Microbiology, Food/Medical/Pharmaceutical/Diagnostic Microbiology and Microbial Biotechnology.


Preface to the Second Edition

Preface to the First Edition


  • World of Microorganisms: An Introduction
  • Laboratory Safety Rules and Regulations
  • Principle and working of Basic Equipment
  • Microscopy and Basic Microbiological Techniques
  • Staining Techniques for Bacteria
  • Biochemical Tests
  • Culture Media Preparation
  • Basic Microbiological Techniques for Culturing Bacteria
  • Methods used for identifying unknown Bacterial Isolates
  • Study of Environmental Influences on Microbial Growth
  • Evaluation of Antimicrobial Agents
  • Microbial Genetics and Molecular Techniques in Biotechnology
  • The Fungi: Molds, Yeasts and Mushrooms
  • Parasitic Protozoa and Helminthes
  • Virology
  • Forest, Horticulture or Agricultural Microbiology
  • Environmental (Soil, Air, Water) Microbiology
  • food Microbiology and Biotechnology
  • Medical Microbiology and Immunology
  • Appendices



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