Practical Manual of Biochemistry [Paper Back]

This course is intended to introduce you to some of the most widely used experimental procedures in biochemistry. The biochemistry practical course, like all practical courses, is an exploration of procedures. In order to get full benefit from the course, you will need to read the manual, and participate as much as possible in the discussions. You also participate in the actual lab work. The more effort you put into the course work, the more you will learn. The class is an opportunity to learn valuable skills. It has been written in easy to understand language with lucid style. This book will serve as a good resource material for medical, dental and paramedical and medical lab technology students.

Section I: Introduction –

1.     Solutions and Buffer,

2.     Quality Control,

3.     Automation,

4.     Laboratory Hazards and Safety,

5.     Specimen Collection and Preservation.

Section II: Qualitative Analysis –

6.     Colour Reactions of Carbohydrates,

7.     Colour Reactions of Proteins,

8.     Colour Reactions of Lipids,

9.     Examination of Urine,

10.   Dry Chemistry Analysis.

Section III: Quantitative Analysis (A) –

11.   Introduction to Volumetric Analysis,

12.   Gastric Juice Analysis,

13.   Estimation of Reducing Sugar in Urine,

14.   Estimation of Serum Calcium,

15.   Estimation of Blood Chloride,

16.   Estimation of Serum Bicarbonate,

17.   Arterial Blood Gas Analysis.

Section IV: Quantitative Analysis (B) –

18.   Colorimetry and Spectrophotometry,

19.   Blood Glucose,

20.   Serum Proteins, Albumin and A/G Ratio,

21.   Serum Creatinine

22.   Blood Urea,

23.   Serum Uric Acid,

24.   Serum Bilirubin,

25.   Serum Cholesterol,

26.   Serum Triglyceride,

27.   Serum Inorganic Phosphate,

28.   Cerebrospinal Fluid (CSF) Analysis.

Section V: Enzyme Assay –

29.   Transaminases (ALT & AST),

30.   Alkaline Phosphatase (ALP),

31.   Acid Phosphatase,

32.   Lactate Dehydrogenase (LDH),

33.   Serum Amylase.

Section VI: Organ Function Tests –

34.   Liver Function Tests (LFT),

35.   Kidney Function Tests (KFT),

36.   Thyroid Function Tests,

37.   Cardiac Function Tests.

Section VII: Techniques –

38.   Sodium and Potassium Estimations by Flame Photometry,

39.   Chemiluminiscence,

40.   Radioimmunoassay (RIA),

41.   Electrophoresis,

42.   Chromatography,

43.   DNA Isolation.

Sadhana Sharma presently Professor and Head of the Department of Biochemistry at All India Institute of Medical Sciences, Patna, Bihar. She is an excellent teacher and researcher with an experience of over 25 years.

Reema Sharma is an Associate Professor in Department of Biochemistry at Santosh Medical College, Ghaziabad, (U.P.). She has been actively involved in teaching medical, dental and paramedical students for last two decades.